• Immunofluorescence analysis of U2OS cells using STAMBP Rabbit polyclonal antibody (STJ29145). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of STAMBP in paraffin-embedded human stomach using STAMBP Rabbit polyclonal antibody (STJ29145) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of STAMBP in paraffin-embedded rat spleen using STAMBP Rabbit polyclonal antibody (STJ29145) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using STAMBP Rabbit polyclonal antibody (STJ29145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STAMBP antibody (100-270) (STJ29145)

SKU:
STJ29145

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STAMBP (100-270) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAMBP
Gene ID: 10617
Uniprot ID: STABP_HUMAN
Immunogen Region: 100-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human STAMBP (NP_998787.1).
Immunogen Sequence: FPKAEELKAELLKRYTKEYT EYNEEKKKEAEELARNMAIQ QELEKEKQRVAQQKQQQLEQ EQFHAFEEMIRNQELEKERL KIVQEFGKVDPGLGGPLVPD LEKPSLDVFPTLTVSSIQPS DCHTTVRPAKPPVVDRSLKP GALSNSESIPTIDGLRHVVV PGRLCPQFLQL
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated after BMP type I receptor activation. Ubiquitinated by SMURF2 in the presence of RNF11.
Function Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
Protein Name Stam-Binding Protein
Associated Molecule With The Sh3 Domain Of Stam
Endosome-Associated Ubiquitin Isopeptidase
Database Links Reactome: R-HSA-5689901
Cellular Localisation Nucleus
Membrane
Peripheral Membrane Protein
Cytoplasm
Early Endosome
Alternative Antibody Names Anti-Stam-Binding Protein antibody
Anti-Associated Molecule With The Sh3 Domain Of Stam antibody
Anti-Endosome-Associated Ubiquitin Isopeptidase antibody
Anti-STAMBP antibody
Anti-AMSH antibody

Information sourced from Uniprot.org

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