• Immunohistochemistry analysis of paraffin-embedded human liver damage using SRGN antibody (STJ29031) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of THP-1 cells, using SRGN antibody (STJ29031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRGN antibody (28-158) (STJ29031)

SKU:
STJ29031

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SRGN (28-158) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRGN
Gene ID: 5552
Uniprot ID: SRGN_HUMAN
Immunogen Region: 28-158
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-158 of human SRGN (NP_002718.2).
Immunogen Sequence: YPTRRARYQWVRCNPDSNSA NCLEEKGPMFELLPGESNKI PRLRTDLFPKTRIQDLNRIF PLSEDYSGSGFGSGSGSGSG SGSGFLTEMEQDYQLVDESD AFHDNLRSLDRNLPSDSQDL GQHGLEEDFML
Post Translational Modifications O-glycosylated.contains chondroitin sulfate and heparan sulfate.
Function Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.
Protein Name Serglycin
Hematopoietic Proteoglycan Core Protein
Platelet Proteoglycan Core Protein
P.pg
Secretory Granule Proteoglycan Core Protein
Database Links Reactome: R-HSA-114608
Cellular Localisation Cytoplasmic Granule
Cytolytic Granule
Secreted
Extracellular Space
Golgi Apparatus
Found In Mast Cell Granules And In Cytoplasmic Granules Of Cytolytic T Lymphocytes From Where It Is Secreted Upon Cell Activation
Secreted Constitutively By Endothelial Cells And Macrophages
Located To Golgi Apparatus During Neutrophil Differentiation
Alternative Antibody Names Anti-Serglycin antibody
Anti-Hematopoietic Proteoglycan Core Protein antibody
Anti-Platelet Proteoglycan Core Protein antibody
Anti-P.pg antibody
Anti-Secretory Granule Proteoglycan Core Protein antibody
Anti-SRGN antibody
Anti-PRG antibody
Anti-PRG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance