| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SRGAP2 (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SRGAP2 |
| Gene ID: | 23380 |
| Uniprot ID: | SRGP2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_056141.2 |
| Specificity: | This antibody is expected to recognize isoform a (NP_056141.2). |
| Immunogen Sequence: | KQEDRQTPRSPDST |
| Function | Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex. Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons. SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses. Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation. Promotes cell repulsion and contact inhibition of locomotion: localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions. In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia. |
| Protein Name | Slit-Robo Rho Gtpase-Activating Protein 2 Srgap2 Formin-Binding Protein 2 Rho Gtpase-Activating Protein 34 |
| Database Links | Reactome: R-HSA-428543 Reactome: R-HSA-5663220 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013406 Reactome: R-HSA-9013420 Reactome: R-HSA-9013423 Reactome: R-HSA-9035034 |
| Cellular Localisation | Cell Membrane Cell Projection Dendritic Spine Postsynaptic Density Postsynaptic Cell Membrane Lamellipodium Cytoplasmic Vesicle Phagosome Nucleus Cytoplasm Cytosol Recruited To Actin-Rich Phagosomes During Phagocytosis Translocates From Nucleus To Cytoplasm During Development |
| Alternative Antibody Names | Anti-Slit-Robo Rho Gtpase-Activating Protein 2 antibody Anti-Srgap2 antibody Anti-Formin-Binding Protein 2 antibody Anti-Rho Gtpase-Activating Protein 34 antibody Anti-SRGAP2 antibody Anti-ARHGAP34 antibody Anti-FNBP2 antibody Anti-KIAA0456 antibody Anti-SRGAP2A antibody |
Information sourced from Uniprot.org