• Western blot analysis of extracts of various cell lines, using SPICE1 antibody (STJ110165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using SPICE1 antibody (STJ110165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPICE1 antibody (1-220) (STJ110165)

SKU:
STJ110165

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPICE1
Gene ID: 152185
Uniprot ID: SPICE_HUMAN
Immunogen Region: 1-220
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1).
Immunogen Sequence: MSFVRVNRCGPRVGVRKTPK VKKKKTSVKQEWDNTVTDLT VHRATPEDLVRRHEIHKSKN RALVHWELQEKALKRKWRKQ KPETLNLEKRRLSIMKEILS DQYQMQDVLEKSDHLIAAAK ELFPRRRTGFPNVTVAPDSS QGPIVVNQDPITQSIFNESV IEPQALNDVDGEEEGTVNSQ SGESENENELDNSLNSQSNT NTDRFLQQLTEENFELISKL
Function Regulator required for centriole duplication, for proper bipolar spindle formation and chromosome congression in mitosis.
Protein Name Spindle And Centriole-Associated Protein 1
Coiled-Coil Domain-Containing Protein 52
Spindle And Centriole-Associated Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Spindle
Alternative Antibody Names Anti-Spindle And Centriole-Associated Protein 1 antibody
Anti-Coiled-Coil Domain-Containing Protein 52 antibody
Anti-Spindle And Centriole-Associated Protein antibody
Anti-SPICE1 antibody
Anti-CCDC52 antibody
Anti-SPICE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance