| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SOX2 |
| Gene ID: | 6657 |
| Uniprot ID: | SOX2_HUMAN |
| Immunogen Region: | 1-317 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human SOX2 (NP_003097.1). |
| Immunogen Sequence: | MYNMMETELKPPGPQQTSGG GGGNSTAAAAGGNQKNSPDR VKRPMNAFMVWSRGQRRKMA QENPKMHNSEISKRLGAEWK LLSETEKRPFIDEAKRLRAL HMKEHPDYKYRPRRKTKTLM KKDKYTLPGGLLAPGGNSMA |
| Post Translational Modifications | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation. Ubiquitinated by WWP2, leading to proteasomal degradation. |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development. |
| Protein Name | Transcription Factor Sox-2 |
| Database Links | Reactome: R-HSA-2892245 Reactome: R-HSA-2892247 Reactome: R-HSA-3769402 Reactome: R-HSA-452723 Reactome: R-HSA-6785807 Reactome: R-HSA-8986944 Reactome: R-HSA-9754189 Reactome: R-HSA-9823739 Reactome: R-HSA-9832991 Reactome: R-HSA-9834899 Reactome: R-HSA-9856649 |
| Cellular Localisation | Nucleus Speckle Cytoplasm Nucleus Acetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic Delocalization Colocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th) Colocalizes With Sox6 In Speckles Colocalizes With Caml In The Nucleus Nuclear Import Is Facilitated By Xpo4 A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Transcription Factor Sox-2 antibody Anti-SOX2 antibody |
Information sourced from Uniprot.org