| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SMO (28-787) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMO |
| Gene ID: |
6608 |
| Uniprot ID: |
SMO_HUMAN |
| Immunogen Region: |
28-787 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 28-787 of human SMO (NP_005622.1). |
| Immunogen Sequence: |
RGAASSGNATGPGPRSAGGS ARRSAAVTGPPPPLSHCGRA APCEPLRYNVCLGSVLPYGA TSTLLAGDSDSQEEAHGKLV LWSGLRNAPRCWAVIQPLLC AVYMPKCENDRVELPSRTLC QATRGPCAIVERERGWPDFL RCTPDRFPEGCTNEVQNIKF NSSGQCEVPLVRTDNPKSWY EDVEGCGIQCQNPLFTEAEH QDMHSY |
| Post Translational Modifications | Phosphorylation by GRK kinases is required for interaction with protein kinase A catalytic subunit PRKACA. |
| Function | G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation. |
| Protein Name | Protein SmoothenedProtein Gx |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5610787Reactome: R-HSA-5620922Reactome: R-HSA-5632684Reactome: R-HSA-5635838 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCilium Localization Is Promoted By Shh And Is Required For Activity |
| Alternative Antibody Names | Anti-Protein Smoothened antibodyAnti-Protein Gx antibodyAnti-SMO antibodyAnti-SMOH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
