• Immunoprecipitation analysis of 300 Mu g extracts of U-87MG cells using 3 Mu g SMC3 antibody (STJ11103048). Western blot was performed from the immunoprecipitate using SMC3 antibody (STJ11103048) at a dilution of 1:1000.
  • Immunofluorescence analysis of C6 cells using SMC3 Rabbit monoclonal antibody (STJ11103048) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SMC3 Rabbit monoclonal antibody (STJ11103048) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using SMC3 Rabbit monoclonal antibody (STJ11103048) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SMC3 Rabbit monoclonal antibody (STJ11103048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SMC3 antibody (1118-1217) [S8MR] (STJ11103048)

SKU:
STJ11103048

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SMC3 (1118-1217) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMC3
Gene ID: 9126
Uniprot ID: SMC3_HUMAN
Immunogen Region: 1118-1217
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1118-1217 of human SMC3 (NP_005436.1).
Immunogen Sequence: GQKSLVALALIFAIQKCDPA PFYLFDEIDQALDAQHRKAV SDMIMELAVHAQFITTTFRP ELLESADKFYGVKFRNKVSH IDVITAEMAKDFVEDDTTHG
Post Translational Modifications Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation. Phosphorylated at Ser-1083 in a SPO11-dependent manner. Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.
Function Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.
Protein Name Structural Maintenance Of Chromosomes Protein 3
Smc Protein 3
Smc-3
Basement Membrane-Associated Chondroitin Proteoglycan
Bamacan
Chondroitin Sulfate Proteoglycan 6
Chromosome-Associated Polypeptide
Hcap
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Chromosome
Centromere
Associates With Chromatin
Before Prophase It Is Scattered Along Chromosome Arms
During Prophase
Most Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By Plk
Except At Centromeres
Where Cohesin Complexes Remain
At Anaphase
The Rad21 Subunit Of The Cohesin Complex Is Cleaved
Leading To The Dissociation Of The Complex From Chromosomes
Allowing Chromosome Separation
The Phosphorylated Form At Ser-1083 Is Preferentially Associated With Unsynapsed Chromosomal Regions
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Protein 3 antibody
Anti-Smc Protein 3 antibody
Anti-Smc-3 antibody
Anti-Basement Membrane-Associated Chondroitin Proteoglycan antibody
Anti-Bamacan antibody
Anti-Chondroitin Sulfate Proteoglycan 6 antibody
Anti-Chromosome-Associated Polypeptide antibody
Anti-Hcap antibody
Anti-SMC3 antibody
Anti-BAM antibody
Anti-BMH antibody
Anti-CSPG6 antibody
Anti-SMC3L1 antibody

Information sourced from Uniprot.org

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