| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-SMC3 (1118-1217) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S8MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMC3 |
| Gene ID: |
9126 |
| Uniprot ID: |
SMC3_HUMAN |
| Immunogen Region: |
1118-1217 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1118-1217 of human SMC3 (NP_005436.1). |
| Immunogen Sequence: |
GQKSLVALALIFAIQKCDPA PFYLFDEIDQALDAQHRKAV SDMIMELAVHAQFITTTFRP ELLESADKFYGVKFRNKVSH IDVITAEMAKDFVEDDTTHG |
| Post Translational Modifications | Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation. Phosphorylated at Ser-1083 in a SPO11-dependent manner. Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin. |
| Function | Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement. |
| Protein Name | Structural Maintenance Of Chromosomes Protein 3Smc Protein 3Smc-3Basement Membrane-Associated Chondroitin ProteoglycanBamacanChondroitin Sulfate Proteoglycan 6Chromosome-Associated PolypeptideHcap |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-2467813Reactome: R-HSA-2468052Reactome: R-HSA-2470946Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-9018519 |
| Cellular Localisation | NucleusChromosomeCentromereAssociates With ChromatinBefore Prophase It Is Scattered Along Chromosome ArmsDuring ProphaseMost Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By PlkExcept At CentromeresWhere Cohesin Complexes RemainAt AnaphaseThe Rad21 Subunit Of The Cohesin Complex Is CleavedLeading To The Dissociation Of The Complex From ChromosomesAllowing Chromosome SeparationThe Phosphorylated Form At Ser-1083 Is Preferentially Associated With Unsynapsed Chromosomal Regions |
| Alternative Antibody Names | Anti-Structural Maintenance Of Chromosomes Protein 3 antibodyAnti-Smc Protein 3 antibodyAnti-Smc-3 antibodyAnti-Basement Membrane-Associated Chondroitin Proteoglycan antibodyAnti-Bamacan antibodyAnti-Chondroitin Sulfate Proteoglycan 6 antibodyAnti-Chromosome-Associated Polypeptide antibodyAnti-Hcap antibodyAnti-SMC3 antibodyAnti-BAM antibodyAnti-BMH antibodyAnti-CSPG6 antibodyAnti-SMC3L1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
