Western blot analysis of lysates from wild type (WT) and SMARCAD1 knockdown (KD) 293T cells using SMARCAD1 Rabbit polyclonal antibody (STJ11105521) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SMARCAD1 antibody (928-1026) (STJ11105521)

SKU:
STJ11105521
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMARCAD1
Gene ID: 56916
Uniprot ID: SMRCD_HUMAN
Immunogen Region: 928-1026
Specificity: Recombinant Protein corresponding to a sequence within amino acids 928-1026 of human SMARCAD1 (NP_064544.2).
Immunogen Sequence: STKAGGLGINLTSANVVILH DIDCNPYNDKQAEDRCHRVG QTKEVLVIKLISQGTIEESM LKINQQKLKLEQDMTTVDEG DEGSMPADIATLLKTSMGL
Tissue Specificity Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).
Function DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.
Protein Name Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1
Atp-Dependent Helicase 1
Hhel1
Cellular Localisation Nucleus
Chromosome
Colocalizes With Pcna At Replication Forks During S Phase
Recruited To Double-Strand Breaks (Dsbs) Sites Of Dna Damage
Alternative Antibody Names Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Containing Dead/H Box 1 antibody
Anti-Atp-Dependent Helicase 1 antibody
Anti-Hhel1 antibody
Anti-SMARCAD1 antibody
Anti-KIAA1122 antibody

Information sourced from Uniprot.org

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