![Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90566/360259/STJ11101652_1__54221.1713124893.jpg?c=1 "Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Smad3 in paraffin-embedded human testis using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90566/360260/STJ11101652_2__42613.1713124894.jpg?c=1 "Immunohistochemistry analysis of Smad3 in paraffin-embedded human testis using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90566/360259/STJ11101652_1__54221.1713124893.jpg?c=1 "Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Protein A |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:20000-1:80000 IHC-P:1:200-1:2000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirem |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMAD3 |
| Gene ID: | 4088 |
| Uniprot ID: | SMAD3_HUMAN |
| Immunogen Region: | 100-200 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Smad3 (NP_005893.1). |
| Immunogen Sequence: | HHELRAMELCEFAFNMKKDE VCVNPYHYQRVETPVLPPVL VPRHTEIPAEFPPLDDYSHS IPENTNFPAGIEPQSNIPET PPPGYLSEDGETSDHQMNHS M |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol. Maximum phosphorylation at the G(1)/S junction. Also phosphorylated on serine residues in the C-terminal SXS motif by TGFBR1 and ACVR1. TGFBR1-mediated phosphorylation at these C-terminal sites is required for interaction with SMAD4, nuclear location and transactivational activity, and appears to be a prerequisite for the TGF-beta mediated phosphorylation in the linker region. Dephosphorylated in the C-terminal SXS motif by PPM1A. This dephosphorylation disrupts the interaction with SMAD4, promotes nuclear export and terminates TGF-beta-mediated signaling. Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Phosphorylated by PDPK1. Acetylation in the nucleus by EP300 in the MH2 domain regulates positively its transcriptional activity and is enhanced by TGF-beta. Poly-ADP-ribosylated by PARP1 and PARP2. ADP-ribosylation negatively regulates SMAD3 transcriptional responses during the course of TGF-beta signaling. Ubiquitinated. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover. Undergoes STUB1-mediated ubiquitination and degradation. |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 3 Mad Homolog 3 Mad3 Mothers Against Dpp Homolog 3 Hmad-3 Jv15-2 Smad Family Member 3 Smad 3 Smad3 Hsmad3 |
| Database Links | Reactome: R-HSA-1181150 Reactome: R-HSA-1502540 Reactome: R-HSA-2173788 Reactome: R-HSA-2173789 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-3304356 Reactome: R-HSA-3311021 Reactome: R-HSA-3315487 Reactome: R-HSA-3656532 Reactome: R-HSA-5689880 Reactome: R-HSA-8941855 Reactome: R-HSA-8952158 Reactome: R-HSA-9008059 Reactome: R-HSA-9013695 Reactome: R-HSA-9615017 Reactome: R-HSA-9617828 Reactome: R-HSA-9735871 Reactome: R-HSA-9754189 Reactome: R-HSA-9796292 Reactome: R-HSA-9823730 Reactome: R-HSA-9839394 |
| Cellular Localisation | Cytoplasm Nucleus Cytoplasmic And Nuclear In The Absence Of Tgf-Beta On Tgf-Beta Stimulation Migrates To The Nucleus When Complexed With Smad4 Through The Action Of The Phosphatase Ppm1a Released From The Smad2/Smad4 Complex And Exported Out Of The Nucleus By Interaction With Ranbp1 Co-Localizes With Lemd3 At The Nucleus Inner Membrane Mapk-Mediated Phosphorylation Appears To Have No Effect On Nuclear Import Pdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm Of The Inner Cell Mass At The Blastocyst Stage |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 3 antibody Anti-Mad Homolog 3 antibody Anti-Mad3 antibody Anti-Mothers Against Dpp Homolog 3 antibody Anti-Hmad-3 antibody Anti-Jv15-2 antibody Anti-Smad Family Member 3 antibody Anti-Smad 3 antibody Anti-Smad3 antibody Anti-Hsmad3 antibody Anti-SMAD3 antibody Anti-MADH3 antibody |
Information sourced from Uniprot.org