• Western blot analysis of lysates from U-87MG cells, using SLC29A1 Rabbit polyclonal antibody (STJ115170) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC29A1 antibody (200-300) (STJ115170)

SKU:
STJ115170

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC29A1 (200-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC29A1
Gene ID: 2030
Uniprot ID: S29A1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC29A1 (NP_001071645.1).
Immunogen Sequence: ELSESAFGYFITACAVIILT IICYLGLPRLEFYRYYQQLK LEGPGEQETKLDLISKGEEP RAGKEESGVSVSNSQPTNES HSIKAILKNISVLAFSVCFI F
Tissue Specificity Expressed in testis at the blood-testis barrier (at protein level). Detected in erythrocytes (at protein level). Expressed at relatively high levels in cerebral cortex, particularly the frontal and parietal lobes, and the thalamus and basal ganglia (at protein level). In the midbrain expressed at moderate levels, whereas in the other areas of the brainstem, namely medulla and pons, cerebellum and the hippocampus expressed at lower amounts when compared to the other brain regions (at protein level). Expressed in Langerhans cells and lymphocytes in the pancreas (at protein level). Expressed in kidney, in polarized renal epithelial cells. Expressed in adipose tissues. Expressed in placenta. Expressed in small intestine.
Post Translational Modifications Glycosylated.
Function Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent transporter. Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine. Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier. Regulates inosine levels in brown adipocytes tissues (BAT) and extracellular inosine levels, which controls BAT-dependent energy expenditure.
Protein Name Equilibrative Nucleoside Transporter 1
Hent1
Equilibrative Nitrobenzylmercaptopurine Riboside-Sensitive Nucleoside Transporter
Equilibrative Nbmpr-Sensitive Nucleoside Transporter
Es Nucleoside Transporter
Nucleoside Transporter - Es-Type
Solute Carrier Family 29 Member 1
Database Links Reactome: R-HSA-83936
Reactome: R-HSA-9748787
Reactome: R-HSA-9755088
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Cell Membrane
Localized To The Basolateral Membrane Of Sertoli Cells
Localized To The Cell Membrane Of Erythrocytes
Alternative Antibody Names Anti-Equilibrative Nucleoside Transporter 1 antibody
Anti-Hent1 antibody
Anti-Equilibrative Nitrobenzylmercaptopurine Riboside-Sensitive Nucleoside Transporter antibody
Anti-Equilibrative Nbmpr-Sensitive Nucleoside Transporter antibody
Anti-Es Nucleoside Transporter antibody
Anti-Nucleoside Transporter - Es-Type antibody
Anti-Solute Carrier Family 29 Member 1 antibody
Anti-SLC29A1 antibody
Anti-ENT1 antibody

Information sourced from Uniprot.org

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