• Western blot analysis of extracts of various cell lines, using SLC22A5 rabbit polyclonal antibody (STJ25566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of HeLa cells using SLC22A5 rabbit polyclonal antibody (STJ25566) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using SLC22A5 rabbit polyclonal antibody (STJ25566) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SLC22A5 rabbit polyclonal antibody (STJ25566) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC22A5 antibody (430-557) (STJ25566)

SKU:
STJ25566

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC22A5 (430-557) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC22A5
Gene ID: 6584
Uniprot ID: S22A5_HUMAN
Immunogen Region: 430-557
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-557 of human SLC22A5 (NP_003051.1).
Immunogen Sequence: VLVMVGKFGVTAAFSMVYVY TAELYPTVVRNMGVGVSSTA SRLGSILSPYFVYLGAYDRF LPYILMGSLTILTAILTLFL PESFGTPLPDTIDQMLRVKG
Tissue Specificity Strongly expressed in kidney, skeletal muscle, heart and placenta. Primarily expressed by surface epithelial cells of the colon (at protein level). Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells. In testis, localized to Sertoli cell basal membranes, peritubular myoid cells and Leydig cells.
Post Translational Modifications Glycosylated. Glycosylation affects the expression levels. Isoform 3: Not glycosylated.
Function Sodium-ion dependent, high affinity carnitine transporter. Involved in the active cellular uptake of carnitine. Transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 11.3. In intestinal epithelia, transports the quorum-sensing pentapeptide CSF (competence and sporulation factor) from Bacillus Subtilis wich induces cytoprotective heat shock proteins contributing to intestinal homeostasis. May also contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (Probable). Isoform 3: Retained in the ER, unable to perform carnitine uptake.
Protein Name Organic Cation/Carnitine Transporter 2
High-Affinity Sodium-Dependent Carnitine Cotransporter
Solute Carrier Family 22 Member 5
Database Links Reactome: R-HSA-200425
Reactome: R-HSA-549127
Reactome: R-HSA-5619053
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Basal Cell Membrane
In Intestinal Cells
Apical Expression Is Induced By Tnf
Localized To The Basal Membrane Of Sertoli Cells
Isoform 3: Endoplasmic Reticulum
Alternative Antibody Names Anti-Organic Cation/Carnitine Transporter 2 antibody
Anti-High-Affinity Sodium-Dependent Carnitine Cotransporter antibody
Anti-Solute Carrier Family 22 Member 5 antibody
Anti-SLC22A5 antibody
Anti-OCTN2 antibody

Information sourced from Uniprot.org

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