| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SLBP (125-223) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLBP |
| Gene ID: |
7884 |
| Uniprot ID: |
SLBP_HUMAN |
| Immunogen Region: |
125-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 125-223 of human SLBP (NP_006518.1). |
| Immunogen Sequence: |
PADFETDESVLMRRQKQINY GKNTIAYDRYIKEVPRHLRQ PGIHPKTPNKFKKYSRRSWD QQIKLWKVALHFWDPPAEEG CDLQEIHPVDLESAESSSE |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated on Thr-61 and Thr-62 in the S-phase. Phosphorylation of Thr-62 by CDK1 primes phosphorylation of Thr-61 by CK2. Phosphorylation of Thr-62 is required for its degradation by the proteasome at the end of the S phase. Its degradation is not required for histone mRNA degradation at the end of the S phase. All the phosphorylated forms detected are present in the cytoplasm. Both unphosphorylated and phosphorylated forms bind the stem-loop structure of histone mRNAs. Phosphorylation at Thr-171 increases affinity for histone mRNAs. Ubiquitinated by the CRL2(FEM1A), CRL2(FEM1B) and CRL2(FEM1C) complexes, leading to its degradation. |
| Function | RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs. |
| Protein Name | Histone Rna Hairpin-Binding ProteinHistone Stem-Loop-Binding Protein |
| Database Links | Reactome: R-HSA-159230Reactome: R-HSA-73856Reactome: R-HSA-77588 |
| Cellular Localisation | CytoplasmNucleusPolyribosome-AssociatedLocalizes Predominantly In The Nucleus At The G1/G2 Phases And The Beginning Of S PhaseThrough The S PhasePartially Redistributes To The CytoplasmBinding To Histone Mrna Is Necessary For Cytoplasmic LocalizationShuttles Between The Nucleus And The CytoplasmImported In The Nucleus By The Importin Alpha/Importin Beta Receptor |
| Alternative Antibody Names | Anti-Histone Rna Hairpin-Binding Protein antibodyAnti-Histone Stem-Loop-Binding Protein antibodyAnti-SLBP antibodyAnti-HBP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
