| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SIAH2 (100-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIAH2 |
| Gene ID: |
6478 |
| Uniprot ID: |
SIAH2_HUMAN |
| Immunogen Region: |
100-200 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SIAH2 (NP_005058.3). |
| Immunogen Sequence: |
VCNQCRQKLSCCPTCRGALT PSIRNLAMEKVASAVLFPCK YATTGCSLTLHHTEKPEHED ICEYRPYSCPCPGASCKWQG SLEAVMSHLMHAHKSITTLQ G |
| Tissue Specificity | Widely expressed at low level. |
| Post Translational Modifications | Phosphorylated at Ser-28 by MAPK14, which mediates the degradation by the proteasome of EGLN3. Phosphorylated at Ser-28 by DYRK2.this increases the ubiquitin ligase activity and promotes degradation of EGLN3. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not. Promotes monoubiquitination of SNCA. Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation. Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance. Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4. Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Siah2Ring-Type E3 Ubiquitin Transferase Siah2Seven In Absentia Homolog 2Siah-2Hsiah2 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-5689880Reactome: R-HSA-977225Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusPredominantly CytoplasmicPartially Nuclear |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Siah2 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Siah2 antibodyAnti-Seven In Absentia Homolog 2 antibodyAnti-Siah-2 antibodyAnti-Hsiah2 antibodyAnti-SIAH2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
