• Western blot analysis of extracts of MCF-7 cells, using SFI1 antibody (STJ110658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SFI1 antibody (972-1211) (STJ110658)

SKU:
STJ110658

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SFI1 (972-1211) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFI1
Gene ID: 9814
Uniprot ID: SFI1_HUMAN
Immunogen Region: 972-1211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2).
Immunogen Sequence: HSARKQPRRPHFLLEPAQSQ RPQKPQEHGLGMAQPAAPSL TRPFLAEAPTALVPHSPLPG ALSSAPGPKQPPTASTGPEL LLLPLSSFMPCGAAAPARVS AQRATPRDKPPVPSSLASVP DPHLLLPGDFSATRAGPGLS TAGSLDLEAELEEIQQQLLH YQTTKQNLWSCRRQASSLRR WLELNREEPGPEDQEVEQQV QKELEQVEMQIQLLAEELQA QRQPIGACVARIQALRQAL
Function Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.
Protein Name Protein Sfi1 Homolog
Hsfi1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Localized Close To The Centriole
Alternative Antibody Names Anti-Protein Sfi1 Homolog antibody
Anti-Hsfi1 antibody
Anti-SFI1 antibody
Anti-KIAA0542 antibody

Information sourced from Uniprot.org

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