• Immunohistochemistry analysis of paraffin-embedded mouse kidney using SF3B3/SAP130 antibody (STJ27899) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using SF3B3/SAP130 antibody (STJ27899) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SF3B3/SAP130 antibody (STJ27899) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SF3B3/SAP130 antibody (STJ27899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.

Anti-SF3B3 antibody (948-1217) (STJ27899)

SKU:
STJ27899

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B3
Gene ID: 23450
Uniprot ID: SF3B3_HUMAN
Immunogen Region: 948-1217
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 948-1217 of human SF3B3/SAP130 (NP_036558.3).
Immunogen Sequence: VPAAIAPFQGRVLIGVGKLL RVYDLGKKKLLRKCENKHIA NYISGIQTIGHRVIVSDVQE SFIWVRYKRNENQLIIFADD TYPRWVTTASLLDYDTVAGA DKFGNICVVRLPPNTNDEVD EDPTGNKALWDRGLLNGASQ KAEVIMNYHVGETVLSLQKT TLIPGGSESLVYTTLSGGIG ILVPFTSHEDHDFFQHVEMH LRSEHPPLCGRDHLSFRSYY FPVKNVIDGDLCEQFNSME
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.
Protein Name Splicing Factor 3b Subunit 3
Pre-Mrna-Splicing Factor Sf3b 130 Kda Subunit
Sf3b130
Staf130
Spliceosome-Associated Protein 130
Sap 130
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 3 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 130 Kda Subunit antibody
Anti-Sf3b130 antibody
Anti-Staf130 antibody
Anti-Spliceosome-Associated Protein 130 antibody
Anti-Sap 130 antibody
Anti-SF3B3 antibody
Anti-KIAA0017 antibody
Anti-SAP130 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance