• Western blot analysis of extracts of various cell lines, using SF3B3/SAP130 rabbit monoclonal antibody (STJ11102510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat heart using SF3B3/SAP130 rabbit monoclonal antibody (STJ11102510) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded mouse testis tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human esophageal using SF3B3/SAP130 rabbit monoclonal antibody (STJ11102510) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded mouse spleen tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using SF3B3/SAP130 rabbit monoclonal antibody (STJ11102510) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded mouse brain tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using SF3B3/SAP130 rabbit monoclonal antibody (STJ11102510) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded rat colon tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded rat spleen tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded human colon carcinoma tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded mouse lung tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded human thyroid cancer tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B3/SAP130 in paraffin-embedded Human lung adenocarcinoma tissue using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using SF3B3/SAP130 Rabbit monoclonal antibody (STJ11102510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SF3B3 antibody (1101-1217) [S0MR] (STJ11102510)

SKU:
STJ11102510

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SF3B3 (1101-1217) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B3
Gene ID: 23450
Uniprot ID: SF3B3_HUMAN
Immunogen Region: 1101-1217
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1101-1217 of human SF3B3/SAP130 (Q15393).
Immunogen Sequence: VLSLQKTTLIPGGSESLVYT TLSGGIGILVPFTSHEDHDF FQHVEMHLRSEHPPLCGRDH LSFRSYYFPVKNVIDGDLCE QFNSMEPNKQKNVSEELDRT PPEVSKKLEDIRTRYAF
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Splicing Factor 3b Subunit 3
Pre-Mrna-Splicing Factor Sf3b 130 Kda Subunit
Sf3b130
Staf130
Spliceosome-Associated Protein 130
Sap 130
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 3 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 130 Kda Subunit antibody
Anti-Sf3b130 antibody
Anti-Staf130 antibody
Anti-Spliceosome-Associated Protein 130 antibody
Anti-Sap 130 antibody
Anti-SF3B3 antibody
Anti-KIAA0017 antibody
Anti-SAP130 antibody

Information sourced from Uniprot.org

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