| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SET (1-277) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SET |
| Gene ID: |
6418 |
| Uniprot ID: |
SET_HUMAN |
| Immunogen Region: |
1-277 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human SET/TAF1 (NP_003002.2). |
| Immunogen Sequence: |
MSAPAAKVSKKELNSNHDGA DETSEKEQQEAIEHIDEVQN EIDRLNEQASEEILKVEQKY NKLRQPFFQKRSELIAKIPN FWVTTFVNHPQVSALLGEED EEALHYLTRVEVTEFEDIKS GYRIDFYFDENPYFENKVLS KEFHLNESGDPSSKSTEIKW KSGKDLTKRSSQTQNKASRK RQHEEPESFFTWFTDHSDAG ADELGEVIKDDIWPNPLQYY LVPDMDDEEGEGEEDDDDD |
| Tissue Specificity | Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor. |
| Post Translational Modifications | Isoform 2 is phosphorylated on Ser-15 and Ser-24. Isoform 2 is acetylated on Lys-11. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. Isoform 2: Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1. |
| Function | Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins.however, isoform 2 specific activity is higher. |
| Protein Name | Protein SetHla-Dr-Associated Protein IiInhibitor Of Granzyme A-Activated DnaseIgaadPhapiiPhosphatase 2a Inhibitor I2pp2aI-2pp2aTemplate-Activating Factor ITaf-I |
| Database Links | Reactome: R-HSA-2299718Reactome: R-HSA-450520 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic ReticulumNucleusNucleoplasmIn The CytoplasmFound Both In The Cytosol And Associated With The Endoplasmic ReticulumThe Set Complex Is Associated With The Endoplasmic ReticulumFollowing Ctl Attack And Cleavage By GzmaMoves Rapidly To The NucleusWhere It Is Found In The NucleoplasmAvoiding The NucleolusSimilar Translocation To The Nucleus Is Also Observed For Lymphocyte-Activated Killer Cells After The Addition Of Calcium |
| Alternative Antibody Names | Anti-Protein Set antibodyAnti-Hla-Dr-Associated Protein Ii antibodyAnti-Inhibitor Of Granzyme A-Activated Dnase antibodyAnti-Igaad antibodyAnti-Phapii antibodyAnti-Phosphatase 2a Inhibitor I2pp2a antibodyAnti-I-2pp2a antibodyAnti-Template-Activating Factor I antibodyAnti-Taf-I antibodyAnti-SET antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
