• Immunofluorescence analysis of HepG2 cells using Alpha-1 Antitrypsin (SERPINA1) Rabbit polyclonal antibody (STJ25480) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Alpha-1 Antitrypsin (SERPINA1) Rabbit polyclonal antibody (STJ25480) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Alpha-1 Antitrypsin (SERPINA1) antibody (STJ25480) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SERPINA1 antibody (156-418) (STJ25480)

SKU:
STJ25480

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINA1 (156-418) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINA1
Gene ID: 5265
Uniprot ID: A1AT_HUMAN
Immunogen Region: 156-418
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 156-418 of human Alpha-1 Antitrypsin (SERPINA1) (NP_000286.3).
Immunogen Sequence: EDVKKLYHSEAFTVNFGDTE EAKKQINDYVEKGTQGKIVD LVKELDRDTVFALVNYIFFK GKWERPFEVKDTEEEDFHVD QVTTVKVPMMKRLGMFNIQH
Tissue Specificity Ubiquitous. Expressed in leukocytes and plasma.
Post Translational Modifications N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Protein Name Alpha-1-Antitrypsin
Alpha-1 Protease Inhibitor
Alpha-1-Antiproteinase
Serpin A1 Cleaved Into - Short Peptide From Aat
Spaat
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-204005
Reactome: R-HSA-381426
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Endoplasmic Reticulum
The S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic Reticulum
Short Peptide From Aat: Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Alpha-1-Antitrypsin antibody
Anti-Alpha-1 Protease Inhibitor antibody
Anti-Alpha-1-Antiproteinase antibody
Anti-Serpin A1 Cleaved Into - Short Peptide From Aat antibody
Anti-Spaat antibody
Anti-SERPINA1 antibody
Anti-AAT antibody
Anti-PI antibody
Anti-PRO0684 antibody
Anti-PRO2209 antibody

Information sourced from Uniprot.org

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