• Western blot analysis of extracts of Rat brain, using SEMA3A antibody (STJ27667) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEMA3A antibody (20-270) (STJ27667)

SKU:
STJ27667

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEMA3A (20-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMA3A
Gene ID: 10371
Uniprot ID: SEM3A_HUMAN
Immunogen Region: 20-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Semaphorin 3A (NP_006071.1).
Immunogen Sequence: ANYQNGKNNVPRLKLSYKEM LESNNVITFNGLANSSSYHT FLLDEERSRLYVGAKDHIFS FDLVNIKDFQKIVWPVSYTR RDECKWAGKDILKECANFIK VLKAYNQTHLYACGTGAFHP ICTYIEIGHHPEDNIFKLEN SHFENGRGKSPYDPKLLTAS LLIDGELYSGTAADFMGRDF AIFRTLGHHHPIRTEQHDSR WLNDPKFISAHLISESDNPE DDKVYFFFRENAIDGEHSG
Tissue Specificity Expressed in the dorsal root ganglia.
Function Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Protein Name Semaphorin-3a
Semaphorin Iii
Sema Iii
Database Links Reactome: R-HSA-399954
Reactome: R-HSA-399955
Reactome: R-HSA-399956
Cellular Localisation Secreted
Alternative Antibody Names Anti-Semaphorin-3a antibody
Anti-Semaphorin Iii antibody
Anti-Sema Iii antibody
Anti-SEMA3A antibody
Anti-SEMAD antibody

Information sourced from Uniprot.org

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