• Western blot analysis of lysates from wild type (WT) and SCYL1 knockout (KO) 293T cells, using [KO Validated] SCYL1 Rabbit polyclonal antibody (STJ11100789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SCYL1 antibody (460-740) (STJ11100789)

SKU:
STJ11100789

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCYL1 (460-740) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCYL1
Gene ID: 57410
Uniprot ID: SCYL1_HUMAN
Immunogen Region: 460-740
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3).
Immunogen Sequence: SASTRHRVLTSAFSRATRDP FAPSRVAGVLGFAATHNLYS MNDCAQKILPVLCGLTVDPE KSVRDQAFKAIRSFLSKLES VSEDPTQLEEVEKDVHAASS PGMGGAAASWAGWAVTGVSS LTSKLIRSHPTTAPTETNIP QRPTPEGVPAPAPTPVPATP TTSGHWETQEEDKDTAEDSS TADRWDDEDWGSLEQEAESV LAQQDDWSTGGQVSRASQVS NSDHKSSKSPESDWSSWEA
Tissue Specificity Ubiquitous.
Function Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology. Has no detectable kinase activity in vitro. Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
Protein Name N-Terminal Kinase-Like Protein
Coated Vesicle-Associated Kinase Of 90 Kda
Scy1-Like Protein 1
Telomerase Regulation-Associated Protein
Telomerase Transcriptional Element-Interacting Factor
Teratoma-Associated Tyrosine Kinase
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Endoplasmic Reticulum-Golgi Intermediate Compartment
Golgi Apparatus
Cis-Golgi Network
Localized To The Endoplasmic Reticulum-Golgi Intermediate And Cis-Golgi In An Arf1-Independent Manner
Isoform 1: Cytoplasm
Cytoplasmic Throughout The Cell Cycle
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Cytoplasmic During Interphase And Centrosomal During Mitosis
It Localizes To The Centrosomes In A Microtubule-Independent Manner
Isoform 6: Nucleus
Alternative Antibody Names Anti-N-Terminal Kinase-Like Protein antibody
Anti-Coated Vesicle-Associated Kinase Of 90 Kda antibody
Anti-Scy1-Like Protein 1 antibody
Anti-Telomerase Regulation-Associated Protein antibody
Anti-Telomerase Transcriptional Element-Interacting Factor antibody
Anti-Teratoma-Associated Tyrosine Kinase antibody
Anti-SCYL1 antibody
Anti-CVAK90 antibody
Anti-GKLP antibody
Anti-NTKL antibody
Anti-TAPK antibody
Anti-TEIF antibody
Anti-TRAP antibody
Anti-HT019 antibody

Information sourced from Uniprot.org

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