• Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike RBD mouse monoclonal antibody antibody (STJ11103175) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike RBD monoclonal antibody (STJ11103175) with a linear range of 0. 78-50ng/mL.
  • Immunofluorescence analysis of 293T cells transfected with SARS-COV-2 Spike RBD protein and untreated 293T cells use SARS-COV-2 Spike RBD mouse monoclonal antibody (STJ11103175) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SARS-CoV-2-Spike RBD antibody [S5MM] (STJ11103175)

SKU:
STJ11103175

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Host: Mouse
Applications: WB/IF
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-SARS-CoV-2-Spike RBD is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MM
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:12000
WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Recombinant fusion protein of SARS-CoV-2 CoV spike RBD.
Immunogen Sequence: Email for sequence
Background This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Information sourced from Uniprot.org

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