• Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP15 Protein, using SARS-CoV-2 NSP15 Rabbit polyclonal antibody (STJ11103254) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SARS-CoV-2-NSP15 antibody (6600-6700) (STJ11103254)

SKU:
STJ11103254

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Host: Rabbit
Applications: WB
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP15 (6600-6700) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 6600-6700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 6600-6700 of coronavirus NSP15 (YP_009724389.1).
Immunogen Sequence: VKGLQPSVGPKQASLNGVTL IGEAVKTQFNYYKKVDGVVQ QLPETYFTQSRNLQEFKPRS QMEIDFLELAMDEFIERYKL EGYAFEHIVYGDFSHSQLGG L
Background The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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