• Human melanoma stained with anti-S-100 antibody using peroxidase0conjugate and DAB chromogen. Note intense nuclear and cytoplasmic staining of melanoma cells.

Anti-S-100 antibody [4C4.9] (STJ180235)


Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human
Short Description: Mouse monoclonal antibody anti-S-100 is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 4C4.9
Conjugation: Unconjugated
Isotype: IgG2a/Kappa
Formulation: Liquid in buffer with protein carrier and preservative
Purification: Affinity purified
Dilution Range: 1:150‐300
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Specificity: Positive control: Melanoma
Immunogen: Purified bovine brain S100 p
Background S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. Ab-1 is excellent for immunohistochemical staining of formalin-fixed, paraffin embedded tissues. S100 protein is highly soluble and may be eluted from frozen tissue during staining tissues.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance