| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RTKN |
| Gene ID: | 6242 |
| Uniprot ID: | RTKN_HUMAN |
| Immunogen Region: | 254-513 |
| Immunogen Sequence: | PLCMTQPTASGTLRVQQAGE MQNWAQVHGVLKGTNLFCYR QPEDADTGEEPLLTIAVNKE TRVRAGELDQALGRPFTLSI SNQYGDDEVTHTLQTESREA LQSWMEALWQLFFDMSQWKQ CCDEIMKIETPAPRKPPQAL AKQGSLYHEMAIEPLDDIAA VTDILTQREGARLETPPPWL AMFTDQPALPNPCSPASVAP APDWTHPLPWGRPRTFSLDA VPPDHSPRARSVAPLPPQR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1). |
| Tissue Specificity | Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines. |
| Function | Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. |
| Protein Name | Rhotekin |
| Database Links | Reactome: R-HSA-5666185Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Rhotekin antibodyAnti-RTKN antibodyAnti-RTKN1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98779/382828/STJ119994_1__38128.1713151329.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
