• Western blot analysis of extracts of various cell lines, using RSPH10B rabbit polyclonal antibody (STJ118636) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RSPH10B antibody (1-300) (STJ118636)

SKU:
STJ118636

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RSPH10B (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSPH10B
Gene ID: 222967
Uniprot ID: R10B1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RSPH10B (NP_775836.3).
Immunogen Sequence: MVKEKKKADKKGEKSARSPS SLSDNLDFSKQDGNTTRQEM SPAGVPLLGMQLNEVKPKKD RQNVQQNEDATQYEESILTK LIVESYEGEKVRGLYEGEGF AAFQGGCTYRGMFSEGLMHG QGTYIWADGLKYEGDFVKNV PMNHGVYTWPDGSMYEGEVV NGMRNGFGMFKCSTQPVSYI GHWCNGKRHGKGSIYYNQEG TCWYEGDWVQNIKKGWGIRC YKSGNIYEGQWEDNMRHGE
Function May function as part of the axonemal radial spoke complex 3 (RS3). Radial spoke complexes are important for ciliary motility.
Protein Name Radial Spoke Head 10 Homolog B
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Alternative Antibody Names Anti-Radial Spoke Head 10 Homolog B antibody
Anti-RSPH10B antibody

Information sourced from Uniprot.org

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