• Immunofluorescence analysis of MCF7 cells using RPA3 antibody (STJ115993).
  • Western blot analysis of extracts of mouse spleen, using RPA3 antibody (STJ115993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RPA3 antibody (6-121) (STJ115993)

SKU:
STJ115993

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPA3 (6-121) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPA3
Gene ID: 6119
Uniprot ID: RFA3_HUMAN
Immunogen Region: 6-121
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 6-121 of human RPA3 (NP_002938.1).
Immunogen Sequence: DLPRSRINAGMLAQFIDKPV CFVGRLEKIHPTGKMFILSD GEGKNGTIELMEPLDEEISG IVEVVGRVTAKATILCTSYV QFKEDSHPFDLGLYNEAVKI IHDFPQFYPLGIVQHD
Post Translational Modifications Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER), probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Protein Name Replication Protein A 14 Kda Subunit
Rp-A P14
Replication Factor A Protein 3
Rf-A Protein 3
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174437
Reactome: R-HSA-176187
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-68962
Reactome: R-HSA-69166
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Replication Protein A 14 Kda Subunit antibody
Anti-Rp-A P14 antibody
Anti-Replication Factor A Protein 3 antibody
Anti-Rf-A Protein 3 antibody
Anti-RPA3 antibody
Anti-REPA3 antibody
Anti-RPA14 antibody

Information sourced from Uniprot.org

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