| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Replication protein A 14 kDa subunit (30-80 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPA3 |
| Gene ID: | 6119 |
| Uniprot ID: | RFA3_HUMAN |
| Immunogen Region: | 30-80 aa |
| Specificity: | The antibody detects endogenous RPA14 |
| Immunogen: | Synthetic peptide from the human protein at the amino acid range 30-80 |
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER), probably through interaction with UNG. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. |
| Protein Name | Replication Protein A 14 Kda Subunit Rp-A P14 Replication Factor A Protein 3 Rf-A Protein 3 |
| Database Links | Reactome: R-HSA-110312 Reactome: R-HSA-110314 Reactome: R-HSA-110320 Reactome: R-HSA-174437 Reactome: R-HSA-176187 Reactome: R-HSA-3371453 Reactome: R-HSA-3371511 Reactome: R-HSA-5358565 Reactome: R-HSA-5358606 Reactome: R-HSA-5651801 Reactome: R-HSA-5655862 Reactome: R-HSA-5656121 Reactome: R-HSA-5656169 Reactome: R-HSA-5685938 Reactome: R-HSA-5685942 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-5696395 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6783310 Reactome: R-HSA-6804756 Reactome: R-HSA-68962 Reactome: R-HSA-69166 Reactome: R-HSA-69473 Reactome: R-HSA-912446 Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Replication Protein A 14 Kda Subunit antibody Anti-Rp-A P14 antibody Anti-Replication Factor A Protein 3 antibody Anti-Rf-A Protein 3 antibody Anti-RPA3 antibody Anti-REPA3 antibody Anti-RPA14 antibody |
Information sourced from Uniprot.org