• Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug RPA2 Rabbit polyclonal antibody antibody (STJ25387 1:100). Western blot was performed from the immunoprecipitate using RPA2 Rabbit polyclonal antibody antibody (STJ25387) at a dilition of 1:1000.
  • Immunofluorescence analysis of C6 cells using RPA2 Rabbit polyclonal antibody (STJ25387) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RPA2 Rabbit polyclonal antibody (STJ25387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPA2 antibody (80-270) (STJ25387)

SKU:
STJ25387

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPA2 (80-270) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPA2
Gene ID: 6118
Uniprot ID: RFA2_HUMAN
Immunogen Region: 80-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).
Immunogen Sequence: IRHAEKAPTNIVYKIDDMTA APMDVRQWVDTDDTSSENTV VPPETYVKVAGHLRSFQNKK SLVAFKIMPLEDMNEFTTHI LEVINAHMVLSKANSQPSAG RAPISNPGMSEAGNFGGNSF MPANGLTVAQNQVLNLIKAC PRPEGLNFQDLKNQLKHMSV SSIKQAVDFLSNEGHIYSTV DDDHFKSTDAE
Post Translational Modifications Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Protein Name Replication Protein A 32 Kda Subunit
Rp-A P32
Replication Factor A Protein 2
Rf-A Protein 2
Replication Protein A 34 Kda Subunit
Rp-A P34
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174437
Reactome: R-HSA-176187
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-68962
Reactome: R-HSA-69166
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Pml Body
Redistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner
Alternative Antibody Names Anti-Replication Protein A 32 Kda Subunit antibody
Anti-Rp-A P32 antibody
Anti-Replication Factor A Protein 2 antibody
Anti-Rf-A Protein 2 antibody
Anti-Replication Protein A 34 Kda Subunit antibody
Anti-Rp-A P34 antibody
Anti-RPA2 antibody
Anti-REPA2 antibody
Anti-RPA32 antibody
Anti-RPA34 antibody

Information sourced from Uniprot.org

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