• Western blot analysis of extracts of various cell lines, using RNF166 antibody (STJ110575) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using RNF166 antibody (STJ110575) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using RNF166 antibody (STJ110575) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RNF166 antibody (STJ110575) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using RNF166 antibody (STJ110575) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RNF166 antibody (STJ110575) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using RNF166 antibody (STJ110575) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RNF166 antibody (STJ110575) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using RNF166 antibody (STJ110575) (40x lens).
  • Western blot analysis of extracts of various cell lines, using RNF166 antibody (STJ110575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-RNF166 antibody (102-201) (STJ110575)

SKU:
STJ110575

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF166 (102-201) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF166
Gene ID: 115992
Uniprot ID: RN166_HUMAN
Immunogen Region: 102-201
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 102-201 of human RNF166 (NP_849163.1).
Immunogen Sequence: NKKVTLAKMRVHISSCLKVQ EQMANCPKFVPVVPTSQPIP SNIPNRSTFACPYCGARNLD QQELVKHCVESHRSDPNRVV CPICSAMPWGDPSYKSANFL
Function E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates. In turn, participates in different biological processes including interferon production or autophagy. Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6. Also plays a role in the early recruitment of autophagy adapters to bacteria. Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication.
Protein Name E3 Ubiquitin-Protein Ligase Rnf166
Ring Finger Protein 166
Ring-Type E3 Ubiquitin Transferase Rnf166
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf166 antibody
Anti-Ring Finger Protein 166 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf166 antibody
Anti-RNF166 antibody

Information sourced from Uniprot.org

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