• Western blot analysis of extracts of various cell lines, using RNF13 antibody (STJ110661) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using RNF13 antibody (STJ110661) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 10s.

Anti-RNF13 antibody (204-381) (STJ110661)

SKU:
STJ110661

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF13 (204-381) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF13
Gene ID: 11342
Uniprot ID: RNF13_HUMAN
Immunogen Region: 204-381
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-381 of human RNF13 (NP_009213.1).
Immunogen Sequence: TKFVQDRHRARRNRLRKDQL KKLPVHKFKKGDEYDVCAIC LDEYEDGDKLRILPCSHAYH CKCVDPWLTKTKKTCPVCKQ KVVPSQGDSDSDTDSSQEEN EVTEHTPLLRPLASVSAQSF GALSESRSHQNMTESSDYEE DDNEDTDSSDAENEINEHDV VVQLQPNGERDYNIANTV
Tissue Specificity Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
Post Translational Modifications Autoubiquitinated.
Function E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization.
Protein Name E3 Ubiquitin-Protein Ligase Rnf13
Ring Finger Protein 13
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Late Endosome Membrane
Lysosome Membrane
Nucleus Inner Membrane
Under Certain Conditions
Relocalizes To Recycling Endosomes And To The Inner Nuclear Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf13 antibody
Anti-Ring Finger Protein 13 antibody
Anti-RNF13 antibody
Anti-RZF antibody

Information sourced from Uniprot.org

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