| Host: | Rabbit |
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF125 |
| Gene ID: | 54941 |
| Uniprot ID: | RN125_HUMAN |
| Immunogen Region: | 1-232 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human RNF125 (NP_060301.2). |
| Immunogen Sequence: | MGSVLSTDSGKSAPASATAR ALERRRDPELPVTSFDCAVC LEVLHQPVRTRCGHVFCRSC IATSLKNNKWTCPYCRAYLP SEGVPATDVAKRMKSEYKNC AECDTLVCLSEMRAHIRTCQ KYIDKYGPLQELEETAARCV CPFCQRELYEDSLLDHCITH HRSERRPVFCPLCRLIPDEN PSSFSGSLIRHLQVSHTLFY DDFIDFNIIEEALIRRVLDR SLLEYVNHSNTT |
| Tissue Specificity | Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells. |
| Post Translational Modifications | Autoubiquitinated, leading to its subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf125 Ring Finger Protein 125 T-Cell Ring Activation Protein 1 Trac-1 |
| Database Links | Reactome: R-HSA-936440 |
| Cellular Localisation | Golgi Apparatus Membrane Lipid-Anchor Shows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi Membrane Not Detected At The Plasma Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibody Anti-Ring Finger Protein 125 antibody Anti-T-Cell Ring Activation Protein 1 antibody Anti-Trac-1 antibody Anti-RNF125 antibody |
Information sourced from Uniprot.org