Anti-RNF11 antibody (1-154) (STJ110626)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF11 (1-154) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF11 |
| Gene ID | 26994 |
| Uniprot ID | RNF11_HUMAN |
| Immunogen Region | 1-154 |
| Immunogen Sequence | MGNCLKSPTSDDISLLHESQ SDRASFGEGTEPDQEPPPPY QEQVPVPVYHPTPSQTRLAT QLTEEEQIRIAQRIGLIQHL PKGVYDPGRDGSEKKIRECV ICMMDFVYGDPIRFLPCMHI YHLDCIDDWLMRSFTCPSCM EPVDAALLSSYETN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human RNF11 (NP_055187.1). |
Additional Info
| Tissue Specificity | Expressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines. |
| Post Translational Modifications | Ubiquitinated in the presence of ITCH, or SMURF2, and UBE2D1, as well as WWP1. Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome. Acylation at both Gly-2 and Cys-4 is required for proper localization to the endosomes. |
| Function | Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome. |
| Protein Name | Ring Finger Protein 11 |
| Database Links | |
| Cellular Localisation | Early EndosomeRecycling EndosomeCytoplasmNucleusPredominantly CytoplasmicWhen UnphosphorylatedAnd NuclearWhen Phosphorylated By Pkb/Akt1 |
| Alternative Antibody Names | Anti-Ring Finger Protein 11 antibodyAnti-RNF11 antibodyAnti-CGI-123 antibody |
Information sourced from Uniprot.org