| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RLIM |
| Gene ID: | 51132 |
| Uniprot ID: | RNF12_HUMAN |
| Immunogen Region: | 535-624 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 535-624 of human RLIM (NP_057204.2). |
| Immunogen Sequence: | FLLNEDDDDQPRGLTKEQID NLAMRSFGENDALKTCSVCI TEYTEGNKLRKLPCSHEYHV HCIDRWLSENSTCPICRRAV LASGNRESVV |
| Tissue Specificity | Expressed in many tissues. |
| Function | E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rlim Lim Domain-Interacting Ring Finger Protein Ring Finger Lim Domain-Binding Protein R-Lim Ring Finger Protein 12 Ring-Type E3 Ubiquitin Transferase Rlim Renal Carcinoma Antigen Ny-Ren-43 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rlim antibody Anti-Lim Domain-Interacting Ring Finger Protein antibody Anti-Ring Finger Lim Domain-Binding Protein antibody Anti-R-Lim antibody Anti-Ring Finger Protein 12 antibody Anti-Ring-Type E3 Ubiquitin Transferase Rlim antibody Anti-Renal Carcinoma Antigen Ny-Ren-43 antibody Anti-RLIM antibody Anti-RNF12 antibody |
Information sourced from Uniprot.org