• Western blot analysis of extracts of various cell lines, using RIPK3 antibody (STJ114965) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of Jurkat cells using RIPK3 rabbit polyclonal antibody (STJ114965) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of RAW264. 7 cells using RIPK3 rabbit polyclonal antibody (STJ114965) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RIPK3 antibody (STJ114965)

SKU:
STJ114965

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RIPK3 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ripk3
Gene ID: 56532
Uniprot ID: RIPK3_MOUSE
Immunogen: Recombinant protein of mouse RIPK3
Immunogen Sequence: Email for sequence
Tissue Specificity Expressed in embryo and in adult spleen, liver, testis, heart, brain and lung.
Post Translational Modifications RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Autophosphorylated following interaction with ZBP1. Phosphorylation of Ser-204 plays a role in the necroptotic function of RIPK3. Autophosphorylates at Thr-231 and Ser-232 following activation by ZBP1: phosphorylation at these sites is a hallmark of necroptosis and is required for binding MLKL. Phosphorylation at Thr-187 is important for its kinase activity, interaction with PELI1 and for its ability to mediate TNF-induced necroptosis. Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation.
Function Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to flavivirus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
Protein Name Receptor-Interacting Serine/Threonine-Protein Kinase 3
Rip-Like Protein Kinase 3
Receptor-Interacting Protein 3
Rip-3
Mrip3
Database Links Reactome: R-MMU-2562578
Reactome: -MMU-3295583
Reactome: -MMU-5213460
Reactome: -MMU-5675482
Reactome: -MMU-937041
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Mainly Cytoplasmic
Present In The Nucleus In Response To Influenza A Virus (Iav) Infection
Alternative Antibody Names Anti-Receptor-Interacting Serine/Threonine-Protein Kinase 3 antibody
Anti-Rip-Like Protein Kinase 3 antibody
Anti-Receptor-Interacting Protein 3 antibody
Anti-Rip-3 antibody
Anti-Mrip3 antibody
Anti-Ripk3 antibody
Anti-Rip3 antibody

Information sourced from Uniprot.org

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