• Western blot analysis of lysates from PC-12 cells, using RICTOR Rabbit polyclonal antibody (STJ11100924) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RICTOR antibody (1043-1708) (STJ11100924)

SKU:
STJ11100924

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RICTOR (1043-1708) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RICTOR
Gene ID: 253260
Uniprot ID: RICTR_HUMAN
Immunogen Region: 1043-1708
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1043-1708 of human RICTOR (NP_689969.2).
Immunogen Sequence: SMFILEDDRFGSSSTSTFFL DINEDTEPTFYDRSGPIKDK NSFPFFASSKLVKNRILNSL TLPNKKHRSSSDPKGGKLSS ESKTSNRRIRTLTEPSVDFN
Post Translational Modifications Phosphorylated by MTOR.when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1.phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. Phosphorylated at Thr-1695 by GSK3A and GSK3B which facilitates RICTOR ubiquitination and subsequent degradation. Ubiquitinated by the SCF(FBXW7) complex, leading to its degradation by the proteasome. Deubiquitinated by USP9X.deubiquitination stabilizes RICTOR and enhances its binding to MTOR, thus promoting mTORC2 complex assembly.
Function Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Protein Name Rapamycin-Insensitive Companion Of Mtor
Avo3 Homolog
Havo3
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-389357
Reactome: R-HSA-5218920
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Alternative Antibody Names Anti-Rapamycin-Insensitive Companion Of Mtor antibody
Anti-Avo3 Homolog antibody
Anti-Havo3 antibody
Anti-RICTOR antibody
Anti-KIAA1999 antibody

Information sourced from Uniprot.org

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