• Western blot analysis of lysates from Mouse brain, using RhoB polyclonal antibody (STJ119169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RHOB antibody (C-Term) (STJ119169)

SKU:
STJ119169

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHOB (C-Term) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOB
Gene ID: 388
Uniprot ID: RHOB_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RhoB (NP_004031.1).
Immunogen Sequence: VPEVKHFCPNVPIILVANKK DLRSDEHVRTELARMKQEPV RTDDGRAMAVRIQAYDYLEC SAKTKEGVREVFETATRAAL QKRYGSQNGCINCCKVL
Post Translational Modifications Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly. (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption.
Function Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
Protein Name Rho-Related Gtp-Binding Protein Rhob
Rho Cdna Clone 6
H6
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-416482
Reactome: R-HSA-416572
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5663220
Reactome: R-HSA-5666185
Reactome: R-HSA-9013026
Cellular Localisation Late Endosome Membrane
Lipid-Anchor
Cell Membrane
Nucleus
Cleavage Furrow
Late Endosomal Membrane (Geranylgeranylated Form)
Plasma Membrane (Farnesylated Form)
Also Detected At The Nuclear Margin And In The Nucleus
Translocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhob antibody
Anti-Rho Cdna Clone 6 antibody
Anti-H6 antibody
Anti-RHOB antibody
Anti-ARH6 antibody
Anti-ARHB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance