| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse/Marmoset |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-RhoA (100-150) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
RHOA |
| Gene ID: |
387 |
| Uniprot ID: |
RHOA_HUMAN |
| Immunogen Region: |
100-150 |
| Specificity: |
Specific for RhOA. |
| Immunogen: |
A synthetic peptide from aa region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan. |
| Function | Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation.promotes KCNA2 endocytosis. Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. May be an activator of PLCE1. In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague. |
| Protein Name | Transforming Protein RhoaRho Cdna Clone 12H12 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-193634Reactome: R-HSA-198203Reactome: R-HSA-209563Reactome: R-HSA-2173791Reactome: R-HSA-392451Reactome: R-HSA-3928662Reactome: R-HSA-3928663Reactome: R-HSA-4086400Reactome: R-HSA-416482Reactome: R-HSA-416550Reactome: R-HSA-416572Reactome: R-HSA-4420097Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5625970Reactome: R-HSA-5627117Reactome: R-HSA-5663220Reactome: R-HSA-5666185Reactome: R-HSA-5689896Reactome: R-HSA-6785631Reactome: R-HSA-6798695Reactome: R-HSA-8849471Reactome: R-HSA-8980692Reactome: R-HSA-8985586Reactome: R-HSA-9013106 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmCytoskeletonCleavage FurrowCell CortexMidbodyCell ProjectionLamellipodiumDendriteNucleusLocalized To Cell-Cell Contacts In Calcium-Treated KeratinocytesTranslocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent MannerLocalizes To The Equatorial Cell Cortex (At The Site Of The Presumptive Furrow) In Early Anaphase In An Activated Form And In A Myosin- And Actin-Independent Manner |
| Alternative Antibody Names | Anti-Transforming Protein Rhoa antibodyAnti-Rho Cdna Clone 12 antibodyAnti-H12 antibodyAnti-RHOA antibodyAnti-ARH12 antibodyAnti-ARHA antibodyAnti-RHO12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
