| Host: | Goat |
| Applications: | WB/IHC-F/IHC-P/IF/ELISA |
| Reactivity: | tdTomato/mCherry/RFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Red Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC-F 1:50-1:500IHC-P 1:50-1:500IF 1:50-1:500ELISA NDLorincz R Alvarez AB Walkey CJ et al. Biomed Pharmacother 2022 Dec PMID: 36587560Hirschberg S Dvorzhak A Rasooli-Nejad S et al. bioRxiv 2020Pan H F |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent p |
| Immunogen: | Purified recombinant peptide produced in E. coli. |
| Immunogen Sequence: | MEQKLISEEDLGTISTMVSK GEEDNMAIIKEFMRFKVHME GSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWD ILSPQFMYGSKAYVKHPADI PDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFI YKVKLRGTNFPSDGPVMQKK TMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKT TYKAKKPVQLPGAYNVNIKL DITSHNEDYTIVEQYERAE |
| Background | Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. Its brightness and emission wavelength makes it ideal for live animal research. |
Information sourced from Uniprot.org