• Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (STJ110791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (STJ110791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-REV1 antibody (120-345) (STJ110791)

SKU:
STJ110791

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-REV1 (120-345) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: REV1
Gene ID: 51455
Uniprot ID: REV1_HUMAN
Immunogen Region: 120-345
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1).
Immunogen Sequence: AGRLLSYIPYQLYTKQSSVQ KGLSFNPVCRPEDPLPGPSN IAKQLNNRVNHIVKKIETEN EVKVNGMNSWNEEDENNDFS FVDLEQTSPGRKQNGIPHPR GSTAIFNGHTPSSNGALKTQ DCLVPMVNSVASRLSPAFSQ EEDKAEKSSTDFRDCTLQQL QQSTRNTDALRNPHRTNSFS LSPLHSNTKINGAHHSTVQG PSSTKSTSSVSTFSKAAPSV PSKPSD
Tissue Specificity Ubiquitous.
Function Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Protein Name Dna Repair Protein Rev1
Alpha Integrin-Binding Protein 80
Aibp80
Rev1-Like Terminal Deoxycytidyl Transferase
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Repair Protein Rev1 antibody
Anti-Alpha Integrin-Binding Protein 80 antibody
Anti-Aibp80 antibody
Anti-Rev1-Like Terminal Deoxycytidyl Transferase antibody
Anti-REV1 antibody
Anti-REV1L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance