| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RET |
| Gene ID: |
5979 |
| Uniprot ID: |
RET_HUMAN |
| Immunogen Region: |
1000-1100 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Ret (NP_066124.1). |
| Immunogen Sequence: |
DISKDLEKMMVKRRDYLDLA ASTPSDSLIYDDGLSEEETP LVDCNNAPLPRALPSTWIEN KLYGMSDPNWPGESPVPLTR ADGTNTGFPRYPNDSVYANW M |
| Post Translational Modifications | Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons. |
| Function | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1. Isoform 1: Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL. |
| Protein Name | Proto-Oncogene Tyrosine-Protein Kinase Receptor RetCadherin Family Member 12Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8853659Reactome: R-HSA-9768919Reactome: R-HSA-9830364Reactome: R-HSA-9830674 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosome MembranePredominantly Located On The Plasma MembraneIn The Presence Of Sorl1 And Gfra1Directed To Endosomes |
| Alternative Antibody Names | Anti-Proto-Oncogene Tyrosine-Protein Kinase Receptor Ret antibodyAnti-Cadherin Family Member 12 antibodyAnti-Proto-Oncogene C-Ret Cleaved Into - Soluble Ret Kinase Fragment - Extracellular Cell-Membrane Anchored Ret Cadherin 120 Kda Fragment antibodyAnti-RET antibodyAnti-CDHF12 antibodyAnti-CDHR16 antibodyAnti-PTC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
