• Western blot analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:5000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:5000, RT, 1h.
  • Western blot analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: Human esophageal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: Human liver cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: mouse kindey, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: Rat kindey, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • Immunohistochemistry analysis of GAPDH. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.
  • ICC analysis of GAPDH red. Sample: HeLa, 4% PFA 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG, 1:300, RT, 1h.

Anti-Recombinant-GAPDH antibody [SR5234] (STJA0005234)

SKU:
STJA0005234-100

£92.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Recombinant-GAPDH is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: SR5234
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:1000-1:2000
IHC/IF (H, M, R) 1:500-1:1000
ICC/IF (H, M, R) 1:500-1:1000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Gapdh
Gene ID: 14433
Uniprot ID: G3P_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Protein Name Glyceraldehyde-3-Phosphate Dehydrogenase
Gapdh
Peptidyl-Cysteine S-Nitrosylase Gapdh
Database Links Reactome: R-MMU-70171
Reactome: -MMU-70263
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Nucleus
Translocates To The Nucleus Following S-Nitrosylation And Interaction With Siah1
Which Contains A Nuclear Localization Signal
Colocalizes With Chp1 To Small Punctate Structures Along The Microtubules Tracks
Alternative Antibody Names Anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody
Anti-Gapdh antibody
Anti-Peptidyl-Cysteine S-Nitrosylase Gapdh antibody
Anti-Gapdh antibody
Anti-Gapd antibody

Information sourced from Uniprot.org

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