| Host: | Rabbit |
| Applications: | IF/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-Recombinant-Cyclin-T1 is suitable for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | RM1E67 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.01M PBS |
| Purification: | Protein A/G purified from cell culture supernatant |
| Dilution Range: | IF: 1:50-1:200 |
| Storage Instruction: | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
| Gene Symbol: | CCNT1 |
| Gene ID: | 904 |
| Uniprot ID: | CCNT1_HUMAN |
| Post Translational Modifications | ADP-ribosylation on serine residues by PARP1 in response to DNA damage disrupts the phase separation activity of CCNT1, thereby preventing activation of CDK9. |
| Function | Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II. (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. |
| Protein Name | Cyclin-T1Cyct1Cyclin-T |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-176034Reactome: R-HSA-2173796Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6807505Reactome: R-HSA-75955Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cyclin-T1 antibodyAnti-Cyct1 antibodyAnti-Cyclin-T antibodyAnti-CCNT1 antibody |
Information sourced from Uniprot.org