• Immunohistochemistry analysis of RCAN1 in paraffin-embedded rat heart using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RCAN1 in paraffin-embedded rat brain using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RCAN1 in paraffin-embedded mouse heart using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RCAN1 in paraffin-embedded mouse brain using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RCAN1 in paraffin-embedded human liver using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RCAN1 in paraffin-embedded human brain using RCAN1 Rabbit polyclonal antibody (STJ27279) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RCAN1 Rabbit polyclonal antibody (STJ27279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RCAN1 antibody (60-245) (STJ27279)

SKU:
STJ27279

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RCAN1 (60-245) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RCAN1
Gene ID: 1827
Uniprot ID: RCAN1_HUMAN
Immunogen Region: 60-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-245 of human RCAN1 (NP_004405.3).
Immunogen Sequence: DLQDLPSATIACHLDPRVFV DGLCRAKFESLFRTYDKDIT FQYFKSFKRVRINFSNPFSA ADARLQLHKTEFLGKEMKLY FAQTLHIGSSHLAPPNPDKQ FLISPPASPPVGWKQVEDAT PVINYDLLYAISKLGPGEKY ELHAATDTTPSVVVHVCESD QEKEEEEEMERMRRPKPKII QTRRPE
Tissue Specificity Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.
Post Translational Modifications Phosphorylation increases its ability to inhibit calcineurin and decreases protein half-life.
Function Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.
Protein Name Calcipressin-1
Adapt78
Down Syndrome Critical Region Protein 1
Myocyte-Enriched Calcineurin-Interacting Protein 1
Mcip1
Regulator Of Calcineurin 1
Alternative Antibody Names Anti-Calcipressin-1 antibody
Anti-Adapt78 antibody
Anti-Down Syndrome Critical Region Protein 1 antibody
Anti-Myocyte-Enriched Calcineurin-Interacting Protein 1 antibody
Anti-Mcip1 antibody
Anti-Regulator Of Calcineurin 1 antibody
Anti-RCAN1 antibody
Anti-ADAPT78 antibody
Anti-CSP1 antibody
Anti-DSC1 antibody
Anti-DSCR1 antibody

Information sourced from Uniprot.org

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