| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RBBP8 |
| Gene ID: | 5932 |
| Uniprot ID: | CTIP_HUMAN |
| Immunogen Region: | 648-897 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 648-897 of human RBBP8 (NP_002885.1). |
| Immunogen Sequence: | VSFENIQWSIDPGADLSQYK MDVTVIDTKDGSQSKLGGET VDMDCTLVSETVLLKMKKQE QKGEKSSNEERKMNDSLEDM FDRTTHEEYESCLADSFSQA ADEEEELSTATKKLHTHGDK QDKVKQKAFVEPYFKGDERE TSLQNFPHIEVVRKKEERRK LLGHTCKECEIYYADMPAEE REKKLASCSRHRFRYIPPNT PENFWEVGFPSTQTCMERGY IKEDLDPCPRPKRRQPYNA |
| Tissue Specificity | Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level). |
| Post Translational Modifications | Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylation at Thr-847 and Thr-859 promote interaction with NBN and recruitment to double-strand breaks (DSBs). Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1. Phosphorylation is not required for tetramerization. Binds to DNA more strongly when dephosphorylated. Ubiquitinated. Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control. Ubiquitinated by RNF138 at its N-terminus. Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex.this modification leads to proteasomal degradation. Ubiquitinated by the E3 FZR1/APC/C complex.this modification leads to proteasomal degradation. |
| Function | Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts: recruited to DSBs by NBN following phosphorylation by CDK1, and promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations. Binds preferentially to DNA Y-junctions and to DNA substrates with blocked ends and promotes intermolecular DNA bridging. |
| Protein Name | Dna Endonuclease Rbbp8 Ctbp-Interacting Protein Ctip Retinoblastoma-Binding Protein 8 Rbbp-8 Retinoblastoma-Interacting Protein And Myosin-Like Rim Sporulation In The Absence Of Spo11 Protein 2 Homolog Sae2 |
| Database Links | Reactome: R-HSA-5685938 Reactome: R-HSA-5685939 Reactome: R-HSA-5685942 Reactome: R-HSA-5693554 Reactome: R-HSA-5693568 Reactome: R-HSA-5693579 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-6804756 Reactome: R-HSA-69473 Reactome: R-HSA-8953750 Reactome: R-HSA-912446 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709570 Reactome: R-HSA-9709603 |
| Cellular Localisation | Nucleus Chromosome Associates With Sites Of Dna Damage In S/G2 Phase Recruited To Dsbs By The Mre11-Rad50-Nbn (Mrn) Complex Following Phosphorylation By Cdk1 Which Promotes Interaction With Nbn Ubiquitinated Rbbp8 Binds To Chromatin Following Dna Damage |
| Alternative Antibody Names | Anti-Dna Endonuclease Rbbp8 antibody Anti-Ctbp-Interacting Protein antibody Anti-Ctip antibody Anti-Retinoblastoma-Binding Protein 8 antibody Anti-Rbbp-8 antibody Anti-Retinoblastoma-Interacting Protein And Myosin-Like antibody Anti-Rim antibody Anti-Sporulation In The Absence Of Spo11 Protein 2 Homolog antibody Anti-Sae2 antibody Anti-RBBP8 antibody Anti-CTIP antibody |
Information sourced from Uniprot.org