Western blot analysis of extracts of various cell lines, using RAPGEF4 antibody (STJ25302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-RAPGEF4 antibody (1-210) (STJ25302)

SKU:
STJ25302
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAPGEF4
Gene ID: 11069
Uniprot ID: RPGF4_HUMAN
Immunogen Region: 1-210
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human RAPGEF4 (NP_001093867.1).
Immunogen Sequence: MLYKKYRQYMAGLLAPPYGV METGSNNDRIPDKENTPLIE PHVPLRPANTITKVPSEKIL RAGKILRNAILSRAPHMIRD RKYHLKTYRQCCVGTELVDW MMQQTPCVHSRTQAVGMWQV LLEDGVLNHVDQEHHFQDKY LFYRFLDDEHEDAPLPTEEE KKECDEELQDTMLLLSQMGP DAHMRMILRKPPGQRTVDDL EIIYEELLHI
Tissue Specificity Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
Function Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.
Protein Name Rap Guanine Nucleotide Exchange Factor 4
Exchange Factor Directly Activated By Camp 2
Exchange Protein Directly Activated By Camp 2
Epac 2
Camp-Regulated Guanine Nucleotide Exchange Factor Ii
Camp-Gefii
Database Links Reactome: R-HSA-354192
Reactome: R-HSA-381676
Reactome: R-HSA-392517
Reactome: R-HSA-422356
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Rap Guanine Nucleotide Exchange Factor 4 antibody
Anti-Exchange Factor Directly Activated By Camp 2 antibody
Anti-Exchange Protein Directly Activated By Camp 2 antibody
Anti-Epac 2 antibody
Anti-Camp-Regulated Guanine Nucleotide Exchange Factor Ii antibody
Anti-Camp-Gefii antibody
Anti-RAPGEF4 antibody
Anti-CGEF2 antibody
Anti-EPAC2 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ191252 Anti-RAPGEF4 antibody (240-320 aa) (STJ191252) Rabbit Polyclonal Human/Rat/Mouse WB/ELISA
STJ11103894 Anti-DYNC1H1 antibody (1-210) (STJ11103894) Rabbit Polyclonal Human WB/ELISA
STJ116804 Anti-KLHL1 antibody (1-210) (STJ116804) Rabbit Polyclonal Human WB/ELISA
STJ11100352 Anti-PDE8B antibody (1-210) (STJ11100352) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ23515 Anti-EIF4A3 antibody (1-210) (STJ23515) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ26212 Anti-WNK1 antibody (1-210) (STJ26212) Rabbit Polyclonal Human WB/IF/ICC/ELISA
STJ28355 Anti-ATF1 antibody (1-210) (STJ28355) Rabbit Polyclonal Human/Mouse WB/IP/ELISA
STJ28011 Anti-ETV7 antibody (1-210) (STJ28011) Rabbit Polyclonal Human WB/ELISA
STJ112352 Anti-KIF16B antibody (1-210) (STJ112352) Rabbit Polyclonal Human WB/ELISA
!