• Western blot analysis of extracts of various cell lines, using Radixin Rabbit monoclonal antibody (STJ11103113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Radixin Rabbit monoclonal antibody (STJ11103113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Radixin antibody (350-450) [S3MR] (STJ11103113)

SKU:
STJ11103113

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Radixin (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDX
Gene ID: 5962
Uniprot ID: RADI_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Radixin (P35241).
Immunogen Sequence: RLKQIEEQTIKAQKELEEQT RKALELDQERKRAKEEAERL EKERRAAEEAKSAIAKQAAD QMKNQEQLAAELAEFTAKIA LLEEAKKKKEEEATEWQHKA F
Post Translational Modifications Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding.
Function Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Protein Name Radixin
Database Links Reactome: R-HSA-437239
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cleavage Furrow
Cell Projection
Microvillus
Highly Concentrated In The Undercoat Of The Cell-To-Cell Adherens Junction And The Cleavage Furrow In The Interphase And Mitotic Phase
Respectively
Alternative Antibody Names Anti-Radixin antibody
Anti-RDX antibody

Information sourced from Uniprot.org

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