| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rad18 (216-495) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD18 |
| Gene ID: |
56852 |
| Uniprot ID: |
RAD18_HUMAN |
| Immunogen Region: |
216-495 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3). |
| Immunogen Sequence: |
INKHLDSCLSREEKKESLRS SVHKRKPLPKTVYNLLSDRD LKKKLKEHGLSIQGNKQQLI KRHQEFVHMYNAQCDALHPK SAAEIVREIENIEKTRMRLE ASKLNESVMVFTKDQTEKEI DEIHSKYRKKHKSEFQLLVD QARKGYKKIAGMSQKTVTIT KEDESTEKLSSVCMGQEDNM TSVTNHFSQSKLDSPEELEP DREEDSSSCIDIQEVLSSSE SDSCNSSSSDIIRDLLEEE |
| Function | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rad18Postreplication Repair Protein Rad18Hhr18Hrad18Ring Finger Protein 73Ring-Type E3 Ubiquitin Transferase Rad18 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With ChromatinColocalizes With Slf1 In The Nucleus And To CentrosomesRelocalizes With Slf1 To Nuclear Foci In Response To Dna DamageAccumulates With The Slf1-Slf2 And Smc5-Smc6 Complexes At Replication-Coupled Dna Interstrand Repair And Dna Double-Strand Breaks (Dsbs) Sites On Chromatin In A Ubiquitin-Dependent Manner |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rad18 antibodyAnti-Postreplication Repair Protein Rad18 antibodyAnti-Hhr18 antibodyAnti-Hrad18 antibodyAnti-Ring Finger Protein 73 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rad18 antibodyAnti-RAD18 antibodyAnti-RNF73 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
