• Immunofluorescence analysis of NIH-3T3 cells using RAB11A Rabbit polyclonal antibody (STJ11104485) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using RAB11A Rabbit polyclonal antibody (STJ11104485) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RAB11A Rabbit polyclonal antibody (STJ11104485) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RAB11A Rabbit polyclonal antibody (STJ11104485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RAB11A antibody (100-200) (STJ11104485)

SKU:
STJ11104485

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ras-Related Protein Rab-11a (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB11A
Gene ID: 8766
Uniprot ID: RB11A_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RAB11A (NP_004654.1).
Immunogen Sequence: ENVERWLKELRDHADSNIVI MLVGNKSDLRHLRAVPTDEA RAFAEKNGLSFIETSALDST NVEAAFQTILTEIYRIVSQK QMSDRRENDMSPSNNVVPIH V
Post Translational Modifications (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Forms a functional Rab11/FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC). Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44.
Protein Name Ras-Related Protein Rab-11a
Rab-11
Yl8
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-432040
Reactome: R-HSA-5620912
Reactome: R-HSA-5620916
Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Cellular Localisation Cell Membrane
Lipid-Anchor
Endosome Membrane
Recycling Endosome Membrane
Cleavage Furrow
Cytoplasmic Vesicle
Phagosome
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network
Localized To Wdr44-Positive Endosomes And Tubules
Translocates With Rab11fip2 From The Vesicles Of The Endocytic Recycling Compartment (Erc) To The Plasma Membrane
During Interphase
Localized In Vesicles Continuously Moving From Peripheral Sorting Endosomes Towards The Pericentrosomal Erc
Localizes To The Cleavage Furrow
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Podxl And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Lumenogenesis
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Localized To Rhodopsin Transport Carriers When Interacting With Rab11afip3 And Asap1 In Photoreceptors
Alternative Antibody Names Anti-Ras-Related Protein Rab-11a antibody
Anti-Rab-11 antibody
Anti-Yl8 antibody
Anti-RAB11A antibody
Anti-RAB11 antibody

Information sourced from Uniprot.org

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