| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PXN (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PXN |
| Gene ID: |
5829 |
| Uniprot ID: |
PAXI_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Paxillin (P49023). |
| Immunogen Sequence: |
MDDLDALLADLESTTSHISK RPVFLSEETPYSYPTGNHTY QEIAVPPPVPPPPSSEALNG TILDPLDQWQPSSSRFIHQQ PQSSSPVYGSSAKTSSVSNP |
| Post Translational Modifications | Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility. |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion. |
| Protein Name | Paxillin |
| Database Links | Reactome: R-HSA-180292Reactome: R-HSA-4420097Reactome: R-HSA-445355Reactome: R-HSA-446343Reactome: R-HSA-446388Reactome: R-HSA-8849471 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionFocal AdhesionCell CortexColocalizes With Integrins At The Cell PeripheryColocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells |
| Alternative Antibody Names | Anti-Paxillin antibodyAnti-PXN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
